Human AMPK (G1/B1/A1) Heterotrimer Protein (His & GST Tag) CT001-H0907B from Sino Biological, Inc.

Description

A DNA sequence encoding the human PRKAG1 (P54619-1) (Met1-Pro331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478-1) (Met1-Ile270), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met1-Gln559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purifie

Product Specs
ItemHuman AMPK (G1/B1/A1) Heterotrimer Protein (His & GST Tag)
CompanySino Biological, Inc.
Price Supplier Page View Company Product Page
Catalog NumberCT001-H0907B
Conjugate/TagN-GST, N-His
Purity> 85 % as determined by SDS-PAGE
TypeRecombinant protein
Molecular WeightThe recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB1 / PRKAA1) has a calculated molecular mass of 160 (38+30+92) KDa. The apparent molecular mass is approximately 38 (PRKAB1), 40 (PRKAG1) & 95 (PRKAA1) KDa respectively in SDS-PAGE under reducing conditions
SpeciesHuman
Molecule NamePRKAG1 & PRKAB1 & PRKAA1
Source/Expression SystemBaculovirus-Insect Cells
NCBI Full Gene Nameprotein kinase AMP-activated catalytic subunit alpha 1, protein kinase AMP-activated non-catalytic subunit beta 1, protein kinase AMP-activated non-catalytic subunit gamma 1
NCBI Gene AliasesAMPK, HAMPKb, AMPK alpha 1, AMPKa1, AMPKG
ActivityThe specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate
FormatSupplied as sterile 20mM Tris, 300mM NaCl, 10% Glycerol, pH 7.5